Kariya Kagetoki

An elite samurai working for the Shogunate known as the "Divine Hand" because of his skill in combat.  According to Goroujuu, a member of the Shogun's council, Kariya has not drawn his swor d in quite some time since there hasn't been a worthy opponent in years. Upon learning of Sara's death, Kariya decides that Mugen and Jin are worthy adversaries, and brings himself out of retirement in order to face them. His calm and collected demeanor hides a cold and manipulative personality. Kariya attempted to gain control of the Mujushin Dojo and is the one responsible for ordering Mariya Enshirou to kill Jin. He is ordered to seek out and kill Kasumi

Seizou, the Sunflower Samurai, for his role in the Shimabara Rebellion as well as Kasumi's family — Fuu. Regarded amongst his peers as "The Hand of the Gods" for his divine skills, being said that there is possibly no one in existence capable of defeating him, he is arguably the strongest fighter in the series. He fights Mugen and Jin simultaneously, easily handling Mugen's fighting style and defeating Jin in a standoff. Similar to Shōryū, he is a master of his ki and is able to use it with great force both offensively and defensively, and to an almost supernatural level. Jin defeats Kariya by sacrificing his own body to breach Kariya's defense, utilizing a strategy that his master, Mariya Enshirou, had described as suicidal.